Multifunctional magnetic bead-based electrochemical immunoassay for the detection of aflatoxin B1 in food
Identifieur interne : 000D99 ( Chine/Analysis ); précédent : 000D98; suivant : 000E00Multifunctional magnetic bead-based electrochemical immunoassay for the detection of aflatoxin B1 in food
Auteurs : RBID : Pascal:09-0474643Descripteurs français
- Pascal (Inist)
- Méthode électrochimique, Méthode immunologique, Nanoparticule, Colorant, Immobilisation, Or, Peroxidase, Anticorps, Traceur, Peroxyde d'hydrogène, Limite détection, Composé ultratrace, Aflatoxine, Produit alimentaire, Hexacyanoferrate, Silice, Sérumalbumine, Oxyde d'indium, Oxyde d'étain, Analyse chimique, Coefficient variation, Technique ELISA, Enrichissement chimique.
- Wicri :
- concept : Colorant, Or, Produit alimentaire.
English descriptors
- KwdEn :
- Aflatoxin, Antibody, Chemical analysis, Chemical enrichment, Detection limit, Dyes, ELISA assay, Electrochemical method, Foodstuff, Gold, Hexacyanoferrates, Hydrogen peroxide, Immobilization, Immunological method, Indium oxide, Nanoparticle, Peroxidase, Serum albumin, Silica, Tin oxide, Tracers, Ultratrace compound, Variation coefficient.
Abstract
A sensitive and reusable electrochemical immunoassay for aflatoxin B1 (AFB1) in food has been developed. A multifunctional magnetic bead (MMB) was initially synthesized using magnetic CoFe2O4 nanoparticle as the core and Prussian blue nanoparticle (PBNP)-doped silica as the shell, and then the prepared MMB was used as an affinity support for the immobilization of the AFB1-bovine serum albumin conjugate (AFB1-BSA). With the aid of an external magnet, the AFB1-BSA-conjugated MMBs were attached on the surface of an indium tin oxide (ITO) electrode. Gold nanoparticles, labeled with horseradish peroxidase (HRP)-bound anti-AFB1 antibodies (HRP-anti-AFB1), were employed as detection antibodies. With a competitive immunoassay format, the concentrations of AFB1 in samples were measured in PBS (pH 7.0) by using PBNP-doped MMBs as the mediator, HRP-anti-AFB1 as the tracer and hydrogen peroxide (H2O2) as the enzyme substrate, and the linear range was 0.05-12 ng/mL with a detection limit of 6.0 pg/mL AFB1 (at 3σ). Intra- and inter-assay coefficients of variation were less than 7.5%. In addition, the content of AFB1 in red paprika specimens has been assayed by the developed immunoassay and a commercially available enzyme-linked immunosorbent assay (ELISA) method, respectively, and consistent results were obtained. The as-prepared immunoassay provides a promising approach for the screening of organic pollutants because it is simple, rapid, highly sensitive, specific, and without the need of sample pre-concentration.
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Pascal:09-0474643Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en" level="a">Multifunctional magnetic bead-based electrochemical immunoassay for the detection of aflatoxin B<sub>1</sub> in food</title><author><name>DIANPING TANG</name><affiliation wicri:level="4"><inist:fA14 i1="01"><s1>Analytical Chemistry, Institute of Hydrochemistry, Technische Universität München, Marchioninistrasse 17</s1><s2>81377 München</s2><s3>DEU</s3><sZ>1 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ></inist:fA14><country>Allemagne</country><placeName><region type="land" nuts="1">Bavière</region><region type="district" nuts="2">District de Haute-Bavière</region><settlement type="city">Munich</settlement></placeName><orgName type="university">Université Louis-et-Maximilien de Munich</orgName></affiliation></author><author><name>ZHAOYANG ZHONG</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Department of Cancer Center, Research Institute of Surgery, Daping Hospital, Third Military Medical University</s1><s2>Chongqing 400042</s2><s3>CHN</s3><sZ>2 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>Chongqing 400042</wicri:noRegion></affiliation></author><author><name sortKey="Niessner, Reinhard" uniqKey="Niessner R">Reinhard Niessner</name><affiliation wicri:level="4"><inist:fA14 i1="01"><s1>Analytical Chemistry, Institute of Hydrochemistry, Technische Universität München, Marchioninistrasse 17</s1><s2>81377 München</s2><s3>DEU</s3><sZ>1 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ></inist:fA14><country>Allemagne</country><placeName><region type="land" nuts="1">Bavière</region><region type="district" nuts="2">District de Haute-Bavière</region><settlement type="city">Munich</settlement></placeName><orgName type="university">Université Louis-et-Maximilien de Munich</orgName></affiliation></author><author><name sortKey="Knopp, Dietmar" uniqKey="Knopp D">Dietmar Knopp</name><affiliation wicri:level="4"><inist:fA14 i1="01"><s1>Analytical Chemistry, Institute of Hydrochemistry, Technische Universität München, Marchioninistrasse 17</s1><s2>81377 München</s2><s3>DEU</s3><sZ>1 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ></inist:fA14><country>Allemagne</country><placeName><region type="land" nuts="1">Bavière</region><region type="district" nuts="2">District de Haute-Bavière</region><settlement type="city">Munich</settlement></placeName><orgName type="university">Université Louis-et-Maximilien de Munich</orgName></affiliation></author></titleStmt><publicationStmt><idno type="inist">09-0474643</idno><date when="2009">2009</date><idno type="stanalyst">PASCAL 09-0474643 INIST</idno><idno type="RBID">Pascal:09-0474643</idno><idno type="wicri:Area/Main/Corpus">004D77</idno><idno type="wicri:Area/Main/Repository">004F52</idno><idno type="wicri:Area/Chine/Extraction">000D99</idno></publicationStmt><seriesStmt><idno type="ISSN">0003-2654</idno><title level="j" type="abbreviated">Analyst : (Lond., 1877, Print)</title><title level="j" type="main">Analyst : (London. 1877. Print)</title></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Aflatoxin</term><term>Antibody</term><term>Chemical analysis</term><term>Chemical enrichment</term><term>Detection limit</term><term>Dyes</term><term>ELISA assay</term><term>Electrochemical method</term><term>Foodstuff</term><term>Gold</term><term>Hexacyanoferrates</term><term>Hydrogen peroxide</term><term>Immobilization</term><term>Immunological method</term><term>Indium oxide</term><term>Nanoparticle</term><term>Peroxidase</term><term>Serum albumin</term><term>Silica</term><term>Tin oxide</term><term>Tracers</term><term>Ultratrace compound</term><term>Variation coefficient</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Méthode électrochimique</term><term>Méthode immunologique</term><term>Nanoparticule</term><term>Colorant</term><term>Immobilisation</term><term>Or</term><term>Peroxidase</term><term>Anticorps</term><term>Traceur</term><term>Peroxyde d'hydrogène</term><term>Limite détection</term><term>Composé ultratrace</term><term>Aflatoxine</term><term>Produit alimentaire</term><term>Hexacyanoferrate</term><term>Silice</term><term>Sérumalbumine</term><term>Oxyde d'indium</term><term>Oxyde d'étain</term><term>Analyse chimique</term><term>Coefficient variation</term><term>Technique ELISA</term><term>Enrichissement chimique</term></keywords><keywords scheme="Wicri" type="concept" xml:lang="fr"><term>Colorant</term><term>Or</term><term>Produit alimentaire</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">A sensitive and reusable electrochemical immunoassay for aflatoxin B<sub>1</sub> (AFB<sub>1</sub>) in food has been developed. A multifunctional magnetic bead (MMB) was initially synthesized using magnetic CoFe<sub>2</sub>O<sub>4</sub> nanoparticle as the core and Prussian blue nanoparticle (PBNP)-doped silica as the shell, and then the prepared MMB was used as an affinity support for the immobilization of the AFB<sub>1</sub>-bovine serum albumin conjugate (AFB<sub>1</sub>-BSA). With the aid of an external magnet, the AFB<sub>1</sub>-BSA-conjugated MMBs were attached on the surface of an indium tin oxide (ITO) electrode. Gold nanoparticles, labeled with horseradish peroxidase (HRP)-bound anti-AFB<sub>1</sub> antibodies (HRP-anti-AFB<sub>1</sub>)<sub>,</sub> were employed as detection antibodies. With a competitive immunoassay format, the concentrations of AFB<sub>1</sub> in samples were measured in PBS (pH 7.0) by using PBNP-doped MMBs as the mediator, HRP-anti-AFB<sub>1</sub> as the tracer and hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) as the enzyme substrate, and the linear range was 0.05-12 ng/mL with a detection limit of 6.0 pg/mL AFB<sub>1</sub> (at 3σ). Intra- and inter-assay coefficients of variation were less than 7.5%. In addition, the content of AFB<sub>1</sub> in red paprika specimens has been assayed by the developed immunoassay and a commercially available enzyme-linked immunosorbent assay (ELISA) method, respectively, and consistent results were obtained. The as-prepared immunoassay provides a promising approach for the screening of organic pollutants because it is simple, rapid, highly sensitive, specific, and without the need of sample pre-concentration.</div></front></TEI><inist><standard h6="B"><pA><fA01 i1="01" i2="1"><s0>0003-2654</s0></fA01><fA02 i1="01"><s0>ANALAO</s0></fA02><fA03 i2="1"><s0>Analyst : (Lond., 1877, Print)</s0></fA03><fA05><s2>134</s2></fA05><fA06><s2>8</s2></fA06><fA08 i1="01" i2="1" l="ENG"><s1>Multifunctional magnetic bead-based electrochemical immunoassay for the detection of aflatoxin B<sub>1</sub> in food</s1></fA08><fA11 i1="01" i2="1"><s1>DIANPING TANG</s1></fA11><fA11 i1="02" i2="1"><s1>ZHAOYANG ZHONG</s1></fA11><fA11 i1="03" i2="1"><s1>NIESSNER (Reinhard)</s1></fA11><fA11 i1="04" i2="1"><s1>KNOPP (Dietmar)</s1></fA11><fA14 i1="01"><s1>Analytical Chemistry, Institute of Hydrochemistry, Technische Universität München, Marchioninistrasse 17</s1><s2>81377 München</s2><s3>DEU</s3><sZ>1 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ></fA14><fA14 i1="02"><s1>Department of Cancer Center, Research Institute of Surgery, Daping Hospital, Third Military Medical University</s1><s2>Chongqing 400042</s2><s3>CHN</s3><sZ>2 aut.</sZ></fA14><fA20><s1>1554-1560</s1></fA20><fA21><s1>2009</s1></fA21><fA23 i1="01"><s0>ENG</s0></fA23><fA43 i1="01"><s1>INIST</s1><s2>1036</s2><s5>354000172580710070</s5></fA43><fA44><s0>0000</s0><s1>© 2009 INIST-CNRS. All rights reserved.</s1></fA44><fA45><s0>44 ref.</s0></fA45><fA47 i1="01" i2="1"><s0>09-0474643</s0></fA47><fA60><s1>P</s1></fA60><fA61><s0>A</s0></fA61><fA64 i1="01" i2="1"><s0>Analyst : (London. 1877. Print)</s0></fA64><fA66 i1="01"><s0>GBR</s0></fA66><fC01 i1="01" l="ENG"><s0>A sensitive and reusable electrochemical immunoassay for aflatoxin B<sub>1</sub> (AFB<sub>1</sub>) in food has been developed. A multifunctional magnetic bead (MMB) was initially synthesized using magnetic CoFe<sub>2</sub>O<sub>4</sub> nanoparticle as the core and Prussian blue nanoparticle (PBNP)-doped silica as the shell, and then the prepared MMB was used as an affinity support for the immobilization of the AFB<sub>1</sub>-bovine serum albumin conjugate (AFB<sub>1</sub>-BSA). With the aid of an external magnet, the AFB<sub>1</sub>-BSA-conjugated MMBs were attached on the surface of an indium tin oxide (ITO) electrode. Gold nanoparticles, labeled with horseradish peroxidase (HRP)-bound anti-AFB<sub>1</sub> antibodies (HRP-anti-AFB<sub>1</sub>)<sub>,</sub> were employed as detection antibodies. With a competitive immunoassay format, the concentrations of AFB<sub>1</sub> in samples were measured in PBS (pH 7.0) by using PBNP-doped MMBs as the mediator, HRP-anti-AFB<sub>1</sub> as the tracer and hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) as the enzyme substrate, and the linear range was 0.05-12 ng/mL with a detection limit of 6.0 pg/mL AFB<sub>1</sub> (at 3σ). Intra- and inter-assay coefficients of variation were less than 7.5%. In addition, the content of AFB<sub>1</sub> in red paprika specimens has been assayed by the developed immunoassay and a commercially available enzyme-linked immunosorbent assay (ELISA) method, respectively, and consistent results were obtained. The as-prepared immunoassay provides a promising approach for the screening of organic pollutants because it is simple, rapid, highly sensitive, specific, and without the need of sample pre-concentration.</s0></fC01><fC02 i1="01" i2="X"><s0>001C04E</s0></fC02><fC02 i1="02" i2="X"><s0>001C04G</s0></fC02><fC03 i1="01" i2="X" l="FRE"><s0>Méthode électrochimique</s0><s5>01</s5></fC03><fC03 i1="01" i2="X" l="ENG"><s0>Electrochemical method</s0><s5>01</s5></fC03><fC03 i1="01" i2="X" l="SPA"><s0>Método electroquímico</s0><s5>01</s5></fC03><fC03 i1="02" i2="X" l="FRE"><s0>Méthode immunologique</s0><s5>02</s5></fC03><fC03 i1="02" i2="X" l="ENG"><s0>Immunological method</s0><s5>02</s5></fC03><fC03 i1="02" i2="X" l="SPA"><s0>Método inmunológico</s0><s5>02</s5></fC03><fC03 i1="03" i2="X" l="FRE"><s0>Nanoparticule</s0><s5>03</s5></fC03><fC03 i1="03" i2="X" l="ENG"><s0>Nanoparticle</s0><s5>03</s5></fC03><fC03 i1="03" i2="X" l="SPA"><s0>Nanopartícula</s0><s5>03</s5></fC03><fC03 i1="04" i2="X" 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l="ENG"><s0>Enzyme</s0><s2>FE</s2></fC07><fC07 i1="03" i2="X" l="SPA"><s0>Enzima</s0><s2>FE</s2></fC07><fC07 i1="04" i2="X" l="FRE"><s0>Toxine</s0></fC07><fC07 i1="04" i2="X" l="ENG"><s0>Toxin</s0></fC07><fC07 i1="04" i2="X" l="SPA"><s0>Toxina</s0></fC07><fN21><s1>341</s1></fN21><fN44 i1="01"><s1>OTO</s1></fN44><fN82><s1>OTO</s1></fN82></pA></standard></inist></record>Pour manipuler ce document sous Unix (Dilib)
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